University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

&nbsp;

The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

&nbsp;

It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Pediocin production by Pediococcus acidilactici was studied in a bio-reactor under two fed batch fermentations (fed batch operation I and fed batch operation II) at constant pH condition using meat processing waste. In fed batch operation II, higher pediocin activity of 4572 AU/ml was achieved comparing to fed batch operation I (pediocin activity of 3657 AU/ml). The kinetics of cell growth was described by two substrates (glucose and protein) model. The correlation between two products (pediocin and lactic acid) and cell growth was described by Luedeking and Piret model. The comparison of simulated data with experimental results obtained from the study was well matched and the validated mathematical model was developed for fed batch processes. © 2020 Scientific Publishers. All rights reserved.

Chemical Engineering

World University root node

Journal of the Indian Chemical Society

Pediocin production by Pediococcus acidilactici in fed batch fermentation using meat processing waste

A study on the adsorption of fluoride was conducted using Zr-treated laterite soil as adsorbent. X-Ray Diffraction analysis (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) were used to characterize the treated laterite soil as adsorbent. Response surface methodology (RSM) was employed to investigate the effect of different operating parameters namely, adsorbent dose, initial concentrations of fluoride and contact time on the uptake of fluoride from aqueous solution. A two level three factor (23) central composite design (CCD) was used for determining the optimum experimental conditions and interactions of process variables. The optimum adsorbent dose, initial concentrations of fluoride and contact time were found to be 2.93 g/L, 40 mg/L and 88.5 min respectively. Fluoride removal was 96.32% at the optimum combination of process parameters. This adsorbent can hence be suggested as an appropriate material for the removal of fluoride.

GENERAL

Removal of fluoride from drinking water by adsorption on Zr-treated laterite soil using response surface methodology

Objectives: The aim of the study was to determine the growth kinetics of Pediococcus acidilactici using a mathematical model for large scale pediocin production. Methods: Growth kinetics of P. acidilactici has been studied for pediocin production in small scale batch fermenter (Erlenmeyer flask) using meat processing waste medium. The experiments have been conducted with varying the concentrations of glucose, protein, and lactic acid. A mathematical model has been developed to describe growth rate, products (pediocin and lactic acid) formation rate, and substrates (glucose and protein) utilization rate. Monod model for dual substrates (glucose and protein) has been used with considering lactic acid inhibition. Luedeking-Piret model has been introduced to describe the production of pediocin and lactic acid. Results: The values of kinetic parameters have been determined using experimental data and model equations. The model prediction has been compared satisfactorily with the experimental data for the validation of the model. Conclusions: The developed model was satisfactorily validated to scale up the production of pediocin. © 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.

Asian Journal of Pharmaceutical and Clinical Research

Study the growth kinetics of pediococcus acidilactici with estimation of kinetic parameters and applied in large scale pediocin production

Objective: Pediocin, a bacteriocin produced by Pediococcus acidilactici NCIM 2292 showed broad inhibitory spectrum. The bacteriocin has sensitivity to proteolytic enzymes, NaCl, pH and temperature. The bacteriocin did not adhere to the surface of the producer cells. Its mode of action appears to be bactericidal, as determined against Listeria monocytogenes MTCC 839 and Staphylococcus aureus NCIM 2127. Methods: Antimicrobial activity of pediocin has been tested against variety of microorganism. It was purified by ammonium sulfate precipitation followed by a Superose 12 fast performance liquid chromatography (FPLC). The purified bacteriocin was treated with proteases, NaCl, pH and temperature. Results: Pediocin showed strong antimicrobial activity against Listeria monocytogenes and Staphylococcus aureus comparing to others orgamigms. Molecular weight of pediocin, estimated by SDS-PAGE was 5.0 kDa. The bacteriocin was inactivated by proteolytic enzymes such as trypsin, proteinase K. chymotrypsin, Pepsin, papain and pronase, but it was active when treated with a-amylase, catalase, lipase and NaCl. Pediocin activity was stable between pH 2.0-8.0 and heat resistant (15 min at 121°C). The bacteriocin was designated as pediocin 2292.Conclusion: Experimental results showed that pediocin 2292 has special antagonism characteristics. The bacteriocin can be successfully utilized as potential bio-preservative in food industry.

International Journal of Pharmacy and Pharmaceutical Sciences

Purification and characterization of pediocin produced by Pediococcus acidilactici NCIM 2292

Goat meat processing waste was enzymatically hydrolyzed using papain to utilize the soluble protein for the growth of Pediococcus acidilactici NCIM 2292 producing pediocin. The suitability of different carbohydrate, namely glucose, lactose, sucrose, maltose and fructose for biomass and bacteriocin production was also investigated. Glucose was observed to be the most preferred carbon source. The experiments based on five-level Central Composite Design (CCD) were conducted to study the effect of hydrolysis time of meat waste, initial pH and initial glucose concentration on the production of biomass and pediocin. The parameters were optimized by Response Surface Methodology (RSM). The optimum values of hydrolysis time, pH and glucose were determined to be 13 h, 6.5 and 20 g/l respectively. The results were analyzed statistically to evaluate analysis of variance (ANOVA). Under optimum condition, the biomass concentration and pediocin activity were measured to be 1.22 g/l and 2285 AU/ml at the end of the exponential growth phase (18 h). Antimicrobial activity of pediocin was assessed by well diffusion method using Staphylococcus aureus NCIM 2127 as indicator organism.

Research Journal of Biotechnology

Optimization of pediocin production by batch fermentation of pediococcus acidilactici NCIM 2292 using goat meat processing waste

This paper deals with the performance of amperometric glucose biosensor on two aspects, namely, use of non-conducting polymers and electron transfer mediator. The proposed method of using number of non conducting polymers for immobilization of enzymes on the surface of an amperometric electrode shows that the performance varies greatly with the type of polymer used. The use of a suitable electron mediator (TBF-TCNQ) also shows a great improvement on performance over the conventional glucose sensors.