Karyotype analysis of three varieties of Indian fennel (Foeniculum vulgare Mill.) revealed that minute structural alteration of chromosomes associated with variation of 4C DNA content, genomic chromosome length and genomic chromosome volume has played an important role in establishment of new cultivated varieties. © 1989, Japan Mendel Society, International Society of Cytology. All rights reserved.

A B DAS

R MALLICK

Fulltext

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

&nbsp;

The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

&nbsp;

It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

CYTOLOGIA

High frequency in vitro regeneration in Holarrhena antidysenterica had been previously achieved successfully using Murashige and Skoog's medium(MS) fortified with 15μM BA. Stability in culture regenerated plants have been observed by studying the changes in chromosome numbers as well as changes in DNA content using cytophotometric data. Somatic chromosomes from regenerated plants revealed a diploid chromosome complement of 2n=22 and confirms the same chromosome number of in vivo plants. No changes in ploidy level were observed which confirm previously reported finding that culture regenerated plants were clonally uniform and lacked somaclonal variation. Stability among regenerants of different stages has also been confirmed using 4C nuclear DNA estimates. Variation in callus morphology and somatic embryo has been observed in culture conditions using various sets of growth regulators. Such changes in morphological patterns have been studied using Scanning Electron Microscopic techniques.

Caryologia

Chromosome stability in culture derived plants of Holarrhena antidysenterica wall, and study of differentiating tissues using SEM

Investigation on karyotype, 4C nuclear DNA amount and interphase nuclear volume (INV) of different Himalayan Bupleurum species belonging to Umbelliferae revealed genetic differentiation. Numerical and structural alternation of chromosomes in interspecific level were manifested in their statistically significant altered species specific 4C nuclear DNA content. Somatic chromosome number ranged between 2n = 14 and 2n = 16. B. himalayense was reported for the first time having 2n = 16 chromosomes. Correlation coefficient among the various chromosomal and nuclear parameters showed no significant progressive or regressive interdependence except in between INV and nuclear DNA amount. Critical differences between 4C DNA content showed interspecific variation. © 1993 Institute of Experimental Botany.

Journal	CYTOLOGIA
Publisher	-
ISSN	0011-4545
Open Access	Yes