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Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India
B. Gaur, , A. Iuliano, S. Rai, A. Krishnan, S. Jain, B. Whitaker, J. Winchell, R. Lal, S. Broor
Published in Wolters Kluwer Medknow Publications
PMID: 31424019
Volume: 37
Issue: 1
Pages: 105 - 108
Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations. © 2019 Indian Society of Anaesthetists. All rights reserved.
About the journal
JournalData powered by TypesetIndian Journal of Medical Microbiology
PublisherData powered by TypesetWolters Kluwer Medknow Publications