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Transformation and expression of a staphylococcal plasmid in Escherichia coli
D SAHA, M BAL
Published in -
1993
Volume: 109
   
Issue: 43499
Pages: 279 - 282
Abstract
A multiple antibiotic-resistant Staphylococcus aureus, was found to possess three plasmid bands in agarose gel electrophoresis. A plasmid of approximately 4.3 kb (pMC790/2) was found to code for ampicillin and tetracycline resistance and to have one EcoRI site when transformed into S. aureus RN 4220. pMC790/2 in unmodified form was transformed into a recA- E. coli at a frequency of 1.2×104 transformants/μg of plasmid DNA. Plasmid (pMC790/2) replicated, maintained itself stably and expressed far better in the E. coli host than in S. aureus. © 1993.
About the journal
JournalFEMS Microbiology Letters
Publisher-
ISSN0378-1097
Open AccessYes