Jute seeds (Corchorus olitorius L. Variety JRO-632) were grown in in vitro in White's medium. The epicotyl expiants were grown in cultured aseptically in both Murashige and Skoog's (MS) and Schenk and Hildebrandt's (SH) basal media with different combinations and concentrations of auxins and cytokinins and in other cases no auxins was utilized. Callus tissues were obtained from the root expiants in MS basal medium supplemented with Napthalene Acetic Acid and coconut milk When the concentrations of Napthalene Acetic Acid were gradually increased vigourous growth of the callus tissues was observed. Napthalene Acetic Acid also enhanced rapid growth of the callus tissues. Cytological analysis revealed that the callus tissue are mixoploid, diploid and polyploid in nature. The present investigation indicates that Napthalene Acetic Acid plays and important role in in vitro on chromosomes of Jute in different ways.