Callus tissues were produced from leaf, stem and seed of Nigella sativa in modified Murashige and Skoog's medium. Depending on the origin of explants, the time of callus initiation and also the properties of the callus tissue were found to be different, especially regarding chromosome instability. © 1980 Birkhäuser Verlag.

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University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Study of callus tissues from different parts ofNigella sativa (Ranunculaceae)

Experientia

Callus cultures were obtained from seeds of Nigella sativa L. on a modified basal medium of Murashige and Skoog (1962). Cytological studies were carried out from the tissues during different periods of growth. Various cytological abnormalities like high and low chromosome number, stickiness, fragmentation, sticky bridges, lagging of chromosomes and multipolarity were observed in all the passages. The way of elimination of extra chromatin material after long cultures has been pointed out. © 1980 Springer-Verlag.

Journal	Experientia
Publisher	BirkhÃ¤user-Verlag
ISSN	0014-4754