Header menu link for other important links
X
Studies on the interaction of chlorpromazine with haemoglobin
M BHATTACHARYYA, U CHAUDHURI, R K PODDAR
Published in -
1990
Volume: 12
   
Issue: 5
Pages: 297 - 301
Abstract
The interaction of chlorpromazine (CPZ), a widely used antipsychotic tranquillizer, with the allosteric protein haemoglobin, has been studied by different methods. From r versus Cf plot obtained by an equilibrium dialysis experiment, the maximum value of r was found to be 6.8 at 0.15 m NaCl. Binding parameters, namely the affinity constant K and the degree of cooperativity nH, were determined from the Hill plot. Circular dichroism studies indicate a conformation change of haemoglobin in the presence of CPZ. Oxygen has been found to be released from haemoglobin with the progressive addition of CPZ. The extent of the release of oxygen depends on the stoichiometric ratio of CPZ: haemoglobin (D/P). The possible nature of the binding site of the protein has been discussed on the basis of the information obtained from fluorescence measurements. © 1990.
About the journal
JournalInternational Journal of Biological Macromolecules
Publisher-
ISSN0141-8130