Studies on the in vitro interaction of β-endorphin with different biomembrane preparations, measured in terms of Ca2+ induced chlorotetracycline (CTC) fluorescence alterations indicate that β-endorphin causes release of calcium ion from both synaptosomal and erythrocyte membrane stores but not from rat brain mitochondria. It seems that the binding pattern or interaction of β-endorphin with synaptosomes and erythrocytes are characteristically different from that of mitochondria, indicating some functional significance of this differential interaction. © 1983.

A K CHAKRABARTI

T K CHATTERJEE

J J GHOSH

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Studies on β-endorphin and membrane-bound calcium interaction using chlorotetracycline (CTC) as a fluorescence probe

Peptides

Chlorotetracycline calcium fluorescence studies have been carried out on acute and chronic capsaicin treated rat lung mitochondria and microsome membrane fractions in the presence of different concentrations of capsaicin (1–100 nM) under in vivo conditions. Results indicate, that acute capsaicin treated rat lung membrane fractions are quite different from those that are chronically treated, with respect to capsaicin mediated calcium permeability, measured in terms of calcium induced CTC fluorescence alterations. It seems that the reported ‘activation’ phenomena of acute capsaicin treatment may be associated with calcium influx across the membrane. On the other hand, the reported ‘desensitization’ phenomena of chronic capsaicin treatment may be due to inhibition of capsaicin mediated calcium influx. Copyright © 1991 John Wiley & Sons, Ltd.

Phytotherapy Research

In vitro and in vivo studies on capsaicin and membrane bound calcium interactions in the rat lung using chlorotetracycline (CTC) as a fluorescence probe

The effect of single, acute (7 pulses/sec., 0.75 volt) and chronic (4 pulses/sec., 0.75 volt) electroacupuncture (EA) treatment on alternate days for a period of 21 days on hepatic functions of rats were studied at cellular and subcellular levels. The points used for EA were Shenshu, Dachangshu and Zusanli. After chronic treatment, protein, RNA, phospholipid, and cholesterol contents of whole liver and liver microsomal fraction increased significantly, liver microsomal G-6 Pase activity increased significantly, microsomal lipid peroxidation value decreased, lipase activity increased. After acute treatment, phospholipid, and cholesterol contents of the whole liver and liver microsomal fraction increased significantly, liver microsomal G-6-Pase activity increased significantly, liver microsomal lipid peroxidation value decreased, GPT and lipase activity of liver increased. The parameters unchanged in acute treatment were as follows: protein, RNA content, GOT activity of the liver, SGOT and SGPT activity, hepatic triglyceride. The parameters unchanged in chronic treatment were as follows: GOT and GPT activity of the liver, SGOT and SGPT activity, hepatic triglyceride. No apparent harmful effect of EA on rat hepatic functions is obvious from present study.The effects of single, acute (7 pulses/sec. , 0. 75 volt) and chronic (4 pulses/sec. , 0. 75 volt) electroacupuncture (EA) treatment on alternate days for a period of 21 days on hepatic functions of rats were studied at cellular and subcellular levels. The points used for EA were Shenshu, Dachangshu and Zusanli. No apparent harmful effect of EA on rat hepatic functions is obvious from present study.

Acupuncture and Electro-Therapeutics Research

Electroacupuncture and its effect on rat hepatic functions

Trends in Biochemical Sciences

Calcium movements in cells

Journal of Neurochemistry

CHLOROTETRACYCLINE AS AN INDICATOR OF THE INTERACTION OF CALCIUM WITH BRAIN MEMBRANE FRACTIONS

Biochimica et Biophysica Acta (BBA) - Biomembranes

Studies on membrane fusion. II. Induction of fusion in pure phospholipid membranes by calcium ions and other divalent metals

American Journal of Psychiatry

Dr. Mendels Replies

Studies on Î²-endorphin and membrane-bound calcium interaction using chlorotetracycline (CTC) as a fluorescence probe

Journal	Peptides
Publisher	-
ISSN	0196-9781