Strain CTA3 was isolated from oil contaminated soil and tested with various petroleum fractions /intermediates viz. gasoline, kerosene, diesel, vacuum gas oil (VGO), lubricating oil base stock (LOBS) as well as some pure hydrocarbons. Based on DNA sequence CTA3 was identified as Acinetobacter junii. The isolated strain CTA3 has been found to be very effective for biodegradation of petroleum fractions at 37° in liquid Bushnell-Haas (BH) media at pH 7. The study reveals that the strain has good potential for degrading higher n-paraffins, above C10.