An experimental study was carried out to examine the biochemical changes in the fermentation broth during L-lysine fermentation by the biotin auxotropic mutant Micrococcus glutamicus AB200. The importance of this experiment is to study the rate of consumption of carbon and nitrogen source with respect to the rate of formation of the product L-lysine. Production of L-lysine was increased up to 72 hours and then declined, pH increases up to 8. 5, dry cell weight showed continuous increased pattern with increased consumption of glucose. Amino nitrogen increases up to 72 hours, then decreased slightly, cell nitrogen and ammonia nitrogen increased continuously where as residual nitrogen decreased sharply.