The aqueous extract of polyherbal Ayurvedic preparation. PD-10 (from the roots of Hemidesmus indicus R. Br. (Asclepiadaceae), Rubia cordifolia L. (Rubiaceae), Cissampelos pareira L. (Menispermaceae); fruits of Terminalia chebula Retz. (Combretaceae), Emblica officinalis Gaertn. (Euphorbiaceae), Terminalia bellirica Roxb. (Combretaceae), Vitis vinifera L. (Vitaceae), Grewia asiatica L. (Tiliaceae), Salvadora persica L. (Salvadoraceae) and granules of Saccharum officinarum L. (Poaceae)) was investigated for antipyretic property. The extract caused significant (p<0.05) antipyretic activity induced pyrexia by using Brewer's yeast in rats. The evaluation of analgesic activity of PD-10 using acetic acid induced writhing model, hot plate method and tail immersion methods in mice revealed very significant (p<0.01) analgesic activity. The ulcerogenicity effect of PD-10 studied at different dosages by Barret's method in rats showed significantly lesser ulcer effect even at very high dosage as compared to that of aspirin. These data confirm the antipyretic, analgesic and ulcerogenic properties of the polyherbal Ayurvedic preparation (PD-10) as enunciated in the traditional texts. © 2008 Asian Network for Scientific Information.