A new water soluble glucan (MGPS), with a molecular weight ∼1.48 × 105 Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography. Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments. The monosaccharide composition analysis showed that MGPS contain only glucose. The backbone of MGPS was composed of two (1 → 3)-β-D-glucopyranosyl, two (1 → 6)-β-D-glucopyranosyl, two (1 → 6)-α-D-glucopyranosyl, and one (1 → 4)-α-D-glucopyranosyl residues, out of which one (1 → 3)-β-D-glucopyranosyl residue was branched at O-6 position with terminal α-D-glucopyranosyl residue and one (1 → 4)-α-D-glucopyranosyl residue branched at O-6 position with terminal β-D-glucopyranosyl residue. In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50 = 390 μg/mL), superoxide radical scavenging activity (IC50 = 70 μg/mL), and ferrous ion chelating activity (IC50 = 290 μg/mL). © 2016 Elsevier Ltd

KRISHNENDU ACHARYA

Botany

P MAITY

A K NANDI

D K MANNA

M PATTANAYAK

I K SEN

S K BHANJA

S SAMANTA

B C PANDA

S PALOI

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Carbohydrate Polymers

A water-soluble heteroglycan (PS) of an average molecular weight ∼1.98 ×105 Da was isolated from the aqueous extract of an edible mushroom Termitomyces clypeatus (R. Heim). The structure of the polysaccharide (PS) was established using total hydrolysis, methylation analysis, Smith degradation, and 1D/2D NMR experiments. Total hydrolysis indicated the presence of d-glucose, d-galactose, d-mannose, and l-fucose in a molar ratio of 4.10:1.95:1.0:0.95, respectively. The chemical and NMR analysis indicated the presence of a repeating unit with a backbone consisting of one each of the residues (1→3)-α-d-galactopyranosyl, (1→3)-α-d-mannopyranosyl, (1→3)-α-d-glucopyranosyl, (1→3)-β-d-glucopyranosyl, (1→6)-β-d-glucopyranosyl, and (1→6)-α-d-galactopyranosyl, respectively. The (1→3)-α-d-mannopyranosyl residue was found branched at O-2 with terminal α-l-fucopyranosyl moiety and (1→3)-β-d-glucopyranosyl residue was branched at O-6 with terminal α-d-glucopyranosyl residue. The PS exhibited antioxidant properties. © 2015 Elsevier Ltd. All rights reserved.

Carbohydrate Research

Heteroglycan of an edible mushroom Termitomyces clypeatus: Structure elucidation and antioxidant properties

A water soluble P-glucan having molecular weight similar to 2 x 10(5) Da was isolated from hot water extract of the fruit bodies of an edible mushroom Entoloma lividoalbum (Kuhner & Romagn) Kubicka. This polysaccharide (ELPS) contains (1 -> 3,6)-beta-D-Glcp, (1 -> 3)-13-beta-Glcp, (1 -> 6)-beta-D-Glcp, and terminal beta-D-Glcp moieties in a molar ratio of nearly 1:1:3:1. Chemical and spectroscopic analysis showed that the backbone of glucan consists of three (1 -> 6)-beta-D-glucopyranosyl and two (1 -> 3)-beta-D-glucopyranosyl residues, out of which one (I 3)-3-D-glucopyranosyl moiety was branched at 0-6 with a terminal p-D-glucopyranosyl residue. This beta-glucan exhibited macrophage, splenocyte, and thymocyte stimulations. It possesses promising antioxidant activities as evidenced from its hydroxyl and superoxide radical scavenging activities and reducing properties. (C) 2015 Elsevier Ltd. All rights reserved.

Structural, immunological, and antioxidant studies of beta-glucan from edible mushroom Entoloma lividoalbum

A water soluble β-glucan (PS) with an average molecular weight ∼1.95 × 105 Da was isolated from the alkaline extract of ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr. and found to consist of terminal, (1 → 3)-, (1 → 6)-, and (1 → 3,6)-linked β-d-glucopyranosyl moieties in a ratio of nearly 1:2:2:1. The structure of this PS was elucidated on the basis of total hydrolysis, methylation analysis, Smith degradation, partial hydrolysis, and 1D/2D NMR experiments. On the basis of these experiments, the repeating unit of the PS was found to contain a backbone of three (1 → 6)-β-d-glucopyranosyl residues, one of which was branched at O-3 position with the side chain consisting of two (1 → 3)-β-d-glucopyranosyl and a terminal β-d-glucopyranosyl residue. This PS showed in vitro macrophage activation by NO production as well as splenocytes and thymocytes proliferation. Moreover, it also exhibited potent antioxidant activities. © 2013 Elsevier Ltd. All rights reserved.

Antioxidant and immunostimulant Î²-glucan from edible mushroom Russula albonigra (Krombh.) Fr.

A water soluble heteroglycan (PS-II) of an average molecular weight ∼5.2 × 104 Da was isolated from the alkaline extract of an edible mushroom Entoloma lividoalbum (Kühner &amp; Romagn) Kubička. Structural characterization of PS-II was carried out using sugar and methylation analysis, periodate oxidation study, and 1D/2D NMR experiments. Sugar analysis indicated the presence of glucose, mannose, galactose, and fucose in a molar ratio of nearly 5:1:2:1. The repeating unit of the PS-II had a backbone consisting of two (1 → 3)-β-d-glucopyranosyl, one (1 → 6)-β-d-glucopyranosyl, one (1 → 2)-α-L-fucopyranosyl, one (1 → 6)-α-d-glucopyranosyl, and two (1 → 6)-α-d- galactopyranosyl residues, out of which one (1 → 3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal β-d-glucopyranosyl residue and one (1 → 6)-α-d-galactopyranosyl residue was branched at O-2 position with terminal β-d-mannopyranosyl residue. PS-II showed ameliorative activities at different concentrations (50, 100, 200, 400 μg/ml) and maintained the redox balance as well as reduced the lipid peroxidation to protect the cell destruction. © 2014 Elsevier Ltd.

Heteroglycan of an edible mushroom Entoloma lividoalbum: Structural characterization and study of its protective role for human lymphocytes

A water soluble branched β-d-glucan (PS-I) with an average molecular weight ~2.1×105Da was isolated from alkaline extract of the fruit bodies of the edible mushroom Entoloma lividoalbum (Kühner &amp; Romagn) Kubička which consists of terminal β-d-glucopyranosyl, (1→3)-β-d-glucopyranosyl, (1→6)-β-d-glucopyranosyl, and (1→3,6)-β-d-glucopyranosyl moieties in a molar ratio of nearly 1:3:2:1. The structure of PS-I was elucidated using acid hydrolysis, methylation analysis, periodate oxidation study, partial hydrolysis, and 1D/2D NMR experiments. The repeating unit of the polysaccharide (PS-I) contains a backbone chain of three (1→6)-β-d-glucopyranosyl residues, one of which was branched at O-3 position with the side chain consisting of three (1→3)-β-d-glucopyranosyl and a terminal β-d-glucopyranosyl residues. Total antioxidant capacity of 1mg PS-I was measured and found equivalent to 70±15μg of ascorbic acid. The PS-I was found to possess hydroxyl and superoxide radical-scavenging activities with EC50 values of 480 and 150μg/mL, respectively. The reducing power of PS-I was determined 0.5 at 480μg/mL. © 2013 .

Journal	Data powered by TypesetCarbohydrate Polymers
Publisher	Data powered by TypesetElsevier Ltd
ISSN	0144-8617
Open Access	No