The aim of this work was to valorization of agricultural waste, locally available jackfruit leaf powder as a bio-adsorbent for removal of Cr(VI) ions from aqueous through continuous column study. Physicochemical and superficial characteristics of jackfruit leaf powder along with Cr(VI) were investigated. FTIR studies revealed that the presence of different functional groups such as carboxyl, hydroxyl and amine groups is responsible for binding of Cr(VI) ions on the adsorbent surface. The performance study of the column was evaluated with respect to variation of important factors like bed depth, initial feed concentration and flow rate. It was found that the flow rate, initial Cr(VI) concentration and the bed depth affect the breakthrough curves. The results showed that the breakthrough time increased with increase in bed height and decrease in flow rates. Different mathematical models viz. Thomas model, Yoon- Nelson model, Bohart-Adams model and Wolborska model were applied to validate the experimental data which fitted reasonably with Thomas, Bohat-Adams, Wolborska and Yoon-Nelson models. The adsorbent was regenerated and found suitable for the reuse. The above findings indicated that jackfruit leaf powder might be used as a good bio-adsorbent for removal of Cr (VI) in continuous column mode.