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Rapid in vitro regeneration and clonal propagation of the fastest growing leguminous tree Albizia falcataria (L.) Fosberg using leaflet explant
N GHOSH, D W SMITH, A CHATTERJEE
Published in Bangladesh Association for Plant Tissue
2010
Volume: 20
   
Issue: 1
Pages: 63 - 72
Abstract
Young leaflets were used as the explants for in vitro regeneration of Albizia falcataria (L.) Fosberg without callus intervention. The leaf explants produced in vitro adventitious shoot buds directly on culturing with MS supplemented with BA, IBA (4.0/0.05 mg/1) and 10% coconut milk (v/v). Addition of casein hydrolysate and coconut milk increased the production of shoot buds. The buds produced shoots and roots and showed 66% survival in a field trial. This technique offers an effective way by which large number of genetically stable plants can be produced, maintained, multiplied and transported as disease free propagules or regenerants, safely and economically.
About the journal
JournalPlant Tissue Culture and Biotechnology
PublisherBangladesh Association for Plant Tissue
ISSN1817-3721
Open AccessYes