In this paper we have studied Left and Right ventricular hypertrophy of heart from ECGs using fractional calculus. An ECG is a rough or unreachable curve which is continuous everywhere but nondifferentiable at some points or all points where classical calculus fails. Our purpose of this paper is to find left and right fractional derivatives at those non-differentiable points and then predict LVH and RVH by calculating the phase transition values (absolute difference of left and right fractional derivatives). Our investigation shows that for LVH patients the phase transition values at the non-differentiable points of V1, V2, V5 and V6 leads are higher than those for normal ECG. For RVH ECG the phase transition values at S are smaller than those of R in V1 leads which are just opposite to the case of normal ECGs. Fractal dimension and Hurst Exponents of V1, V2, V5 and V6 leads of the ECGs have been calculated for both problematic and normal ECGs. All such measures may help doctors to diagnose LVH and RVH from ECG more accurately than the technique they use. ©2020 L&H Scientific Publishing, LLC. All rights reserved. Email address: uttam math@yahoo.co.in