The relationship between spontaneous liquefaction and the characteristics of ejaculated human semen has been investigated. The liquefaction time of ejaculates was found to be significantly correlated with their extent of coagulation and some non‐enzymic components of seminal plasma. Human ejaculates were classified into 3 distinct groups depending on their liquefaction time, and the groups were characterized physico‐chemically. The liquefaction time also revealed a low but significant negative correlation with semen volume. It is concluded that an individual's ejaculatory characteristics can be evaluated simply by determining its liquefaction time. Copyright © 1985, Wiley Blackwell. All rights reserved

A MANDAL

A K BHATTACHARYYA

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

International Journal of Andrology

Prolactin and α‐1,4‐glucosidase levels in seminal plasma were measured in poorly coagulated (I), deficiently coagulated (II) and normally coagulated (III and IV) human ejaculates having 0–20%, 21–50% and 51–100% coagulum respectively 4 min after emission. The prolactin concentration (ng ml‐1, mean ± SEM) in poorly coagulated (5.2 ± 0.48) and deficiently coagulated (7.6 ± 0.72) samples was significantly lower than in the normally coagulated groups III (51–75% coagulum, 8.2 ± 0.43) and IV (76–100% coagulum, 9.9 ± 0.59) as well as the presumably fertile samples (9.2 ± 0.74). A highly significant positive correlation was observed between the profactin level and the percentage coagulum of the ejaculates (r = 0.686, n=58, P&lt;0.001). In contrast, the epididymal marker, α‐glucosidase showed no relationship to seminal coagulation. Copyright © 1991, Wiley Blackwell. All rights reserved

Fulltext

Prolactin and Î±â€1, 4â€glucosidase activity in normal and poorly coagulated human semen

Intra-individual inter-ejaculate variations in the amounts of protein, fructose, N-acetylamino sugar, phosphate, sialic acid and amino sugar in washed coagulum from normal ejaculates of men were highly consistent (N = 9). All the prostatic components studied (acid phosphatase, zinc, calcium and citric acid), except zinc, in the washed coagulum were reduced by 90% of their values in semen (N = 5). The seminal vesicular markers (fructose, N-acetylamino sugar and phosphate) had no association with the coagulum structure and represented the soluble components (N = 5). The concentrations of protein and zinc in the coagulum were higher than those of semen by 114% and 32% respectively. The coagulum contained sialic acid and amino sugar as integrated components.

Journal of Reproduction and Fertility

Biochemical composition of washed human seminal coagulum in comparison to sperm-free semen from the same donors

The purpose of this study was to compare various seminal plasma parameters in fresh human ejaculates exhibiting different amounts of coagulum. Poorly coagulating samples demonstrated significantly lower osmolality and buffering capacity but had a higher pH than did samples with good coagulation. However, no correlation was obtained between the activity of superoxide dismutase and the amount of coagulum. Eight hours after ejaculation, sperm motility had decreased by 15 and 80% in samples with good and poor coagulation, respectively. It is suggested that subfertility may be associated with poor coagulation of ejaculates. Copyright © 1988, Wiley Blackwell. All rights reserved

Differences in osmolality, pH, buffering capacity, superoxide dismutase and maintenance of sperm motility in human ejaculates according to the degree of coagulation

Abnormality in liquefaction of human semen is associated with poor scoring in postcoital tests and may lead to subfertility. A relative study on the seminal plasma characteristics of slowly and normally liquefying human ejaculates has been made. A multitude of seminal vesicular and prostatic components in the seminal plasma of eight slow-liquefying ejaculates from 4 men were compared with ten normally liquefying ejaculates from different healthy volunteers. For 60% liquefaction, the time taken by these two types of ejaculates was 46 and 7 min, respectively. The levels of the components that are mainly secreted by the seminal vesicles were similar in both the groups. But all the prostatic components demonstrated significantly (p < 0.001) decreased levels in the slow-liquefying group from those in the group being compared. It was concluded that relative decrease in the prostatic activity with respect to that of the seminal vesicles appears to be the cause of slow-liquefaction. © 1988 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Systems Biology in Reproductive Medicine

Biochemical parameters of slowly liquefying human ejaculates

Variations in the coagulation-liquefaction property of human seminal coagula have rarely been correlated to their ingredients. A number of seminal components were evaluated in relation to the degree of coagulation and the liquefaction time of human ejaculates. Correlation analyses suggested that the vesicular coagulatory activity can better be assessed by the phosphate level rather than the fructose and N-acetylamino sugar levels. The results showed that the fresh ejaculates with a smaller amount of coagulum possess significantly higher prostatic but lower vesicular activities in comparison with that of the better coagulating samples. The study also revealed that liquefaction time-based fractionation of the whole ejaculate seems to give an idea about the origin of some seminal components like split ejaculation technique. It was concluded that the material characteristics of human ejaculate can be approximated simply by determining its degree of coagulation or liquefaction time. © 1987 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Phosphate, zinc, calcium, citric acid, and acid phosphatase in human ejaculates as related to coagulation/liquefaction

Andrologia

Fructose, Proteins and Coagulation in Human Seminal Plasma

Archives of Andrology

Sialic Acid Content of Human Spermatozoa and Seminal Plasma in Relation to Sperm Counts

Journal of Andrology

Biochemical Aspects of the Coagulation and Liquefaction of Human Semen

Fertility and Sterility

A New Technique to Measure the Liquefaction Rate of Human Semen: The Bag Method**Supported by National Institutes of Health Grants HD 09868 and HD 06315, and Ford Foundation Grant 690-0108A.

Journal	International Journal of Andrology
Publisher	-
ISSN	0105-6263