— A highly nutritional protein coding gene AmA1 of Amaranthus hypochondriacus was searched in different species of Amaranthus by using various molecular biological techniques. PCR, Southern hybridization, primed in situ labeling (PRINS) and Cycling-primed in situ labeling (C-PRINS) confirmed the presence of AmA1 coding region only in Amaranthus hypochondriacus. The AmA1 gene was successfully localized on interphase nuclei and also on the metaphase chromosomes. The single copy, unique AmA1 gene was found to be present in the subtelomeric region of two homologous chromosomes in the group of 2-2.2 μm in length. In the present study, a rapid and reliable method for physical localization of single gene on plant chromosome was described and both the methods for in situ localization i.e. PRINS and C-PRINS were compared. © 2008 Taylor & Francis Group, LLC.

R TUI

S C ROY

Fulltext

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

&nbsp;

The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

&nbsp;

It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Physical localization of highly nutritional protein coding AmA1 gene in amaranths through molecular cytogenetic tools

Caryologia

Mannose binding Allium sativum leaf agglutinin (ASAL) has been shown to be antifeedant and insecticidal against sap-sucking insects. In the present investigation, ASAL coding sequence was expressed under the control of CaMV35S promoter in a chimeric gene cassette containing plant selection marker, hpt and gusA reporter gene of pCAMBIA1301 binary vector in an elite indica rice cv. IR64. Many fertile transgenic plants were generated using scutellar calli as initial explants through Agrobacterium-mediated transformation technology. GUS activity was observed in selected calli and in mature plants. Transformation frequency was calculated to be ∼12.1% ± 0.351 (mean ±SE). Southern blot analyses revealed the integration of ASAL gene into rice genome with a predominant single copy insertion. Transgene localization was detected on chromosomes of transformed plants using PRINS and C-PRINS techniques. Northern and western blot analyses determined the expression of transgene in transformed lines. ELISA analyses estimated ASAL expression up to 0.72 and 0.67% of total soluble protein in T0 and T1 plants, respectively. Survival and fecundity of brown planthopper and green leafhopper were reduced to 36% (P&lt; 0.01), 32% (P&lt; 0.05) and 40.5, 29.5% (P &lt;0.001), respectively, when tested on selected plants in comparison to control plants. Specific binding of expressed ASAL to receptor proteins of insect gut was analysed. Analysis of T1 progenies confirmed the inheritance of the transgenes. Thus, ASAL promises to be a potential component in insect resistance rice breeding programme. © Springer-Verlag 2006.

Planta

Transgenic rice expressing Allium sativum leaf lectin with enhanced resistance against sap-sucking insect pests

Bioinformation

Proteomic characterization of oyster shell organic matrix proteins (OMP)

Hereditas

Towards an Integration of the Physical and the Genetic Chromosome Maps of Barley by in Situ Hybridization

Chromosome Painting

Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS

Proceedings of the National Academy of Sciences

Increased nutritive value of transgenic potato by expressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus

Physical localization of highly nutritional protein coding ama1 gene in amaranths through molecular cytogenetic tools

Journal	Caryologia
Publisher	UNIV FLORENCE BOTANY INST
ISSN	0008-7114
Open Access	Yes