The isolation of pentacyclic triterpenoids from seven species of fresh mangrove leaves using a simple and rapid method is described. The leaves were homogenized using chloroform-methanol and the extract was diluted with water to precipitate out triterpenoids which were separated into neutral and acidic fractions. These were analysed by gas-liquid chromatography as acetyl and trimethylsilyl ether derivatives on a 3% OV-17 column. Sterols were isolated from the chloroform layer by preparative thin layer chromatography and were analysed by gas-liquid chromatography as their trimethylsilyl ether derivatives on a 3% OV-17 column. The triterpenoids found were α-amyrin, β-amyrin, lupeol, oleanolic acid and ursolic acid in most of the samples. Sterols found in all the samples were cholesterol, campesterol, stigmasterol, sitosterol and stigmast-7-en-3β-ol. Retention indices of the triterpenoids and sterols have been determined. © 1985.

A GHOSH

S MISRA

A K DUTTA

A CHOUDHURY

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Pentacyclic triterpenoids and sterols from seven species of mangrove

Phytochemistry

Porteresia coarctata collected, at Prentice and Chuksar Islands of the Sunderban mangrove ecosystem, was studied for its lipid composition. Hydrocarbon, wax ester, sterol esters, triacylglycerols, sterols and terpenoids, were all found in higher proportions in the Prentice Island sample. Palmitic acid was the major fatty acid in all fractions except for the steryl esters of P. coarctata collected from Prentice Island, where oleic and linoleic acids were the major components. Sterols found were, sitosterol, stigmasterol, campesterol and cholesterol, of which, sitosterol and stigmasterol were the major components. Terpenoids present were, β-amyrin, α-amyrin, lupeol, betulin, oleanolic acid and ursolic acid, of which, lupeol and oleanolic acid were the major components. Quantitative variations of the lipid constituents were found between the two samples studied. © 1988.

Lipid composition of Porteresia coarctata from two different mangrove habitats in India

Mangrove plant leaves of Avicennia officinalis, Acanthus illicifolius and Bruguiera gymnorhiza, grown under tidal water stress, were found to biosynthesize higher proportions of hydrocarbons, wax esters, sterol esters, triglycerides, sterols and low-M, terpenes, compared to the plants grown normally. Hydrocarbons and n-alcohols of wax esters, with longer chain lengths were found in higher proportions in the plants grown normally. Higher proportions of unsaturated n-alcohols of wax esters were present in the leaves of periodically submerged plants. Proportions of unsaturated fatty acids of wax esters, sterol esters and triglycerides were also higher in the submerged plants. Sitosterol, stigmasterol and campesterol of the sterol esters, were higher in submerged plants, whereas 28-isofucosterol was higher in normal plants. Of the free sterols, sitosterol was higher in submerged plants, whereas stigmast-7-en-3β-ol and campesterol were higher in normal plants and 28-isofucosterol was found only in submerged plants. Pentacyclic triterpenoids were found only in normal plants, whereas, the submerged plants contained low-M, terpenes in higher proportions. © 1986.

Effect on the leaf lipids of three species of mangrove of periodic submergence in tidal water

The hydrolysis of steryl esters on thin-layer chromatographic plates by porcine pancreatic lipase is described. The sterols and fatty acids produced were separated on the same plate, recovered, and analysed by gas-liquid chromatography for their compositions. Synthetic cholesteryl esters containing various saturated and unsaturated fatty acids and synthetic steryl oleates with various sterols were lipolysed along with steryl esters of Acanthus ilicifolius, Bruguiera gymnorhiza and Rhizophora mucronata mangrove leaves. The major sterol was sitosterol which was accompanied by cholesterol, campesterol, stigmasterol and 28-isofucosterol. In addition, stigmast-7-en-3β-ol was present in R. mucronata leaves. The component fatty acids found in all three species were 16:0, 18:0, 18:1, 18:2 and 18:3. The relative proportions of the sterols and fatty acids were significantly different from the chemotaxonomic standpoint. The results obtained by carrying out plate lipolysis for 45 min at 40° compared well with those produced by conventional chemical hydrolysis. © 1984.

Sterols and fatty acids from three species of mangrove

The fatty acid, sterol and hydrocarbon compositions of oil produced by a new strain of Rhodotorula glutinis isolated from soil and grown on molasses, were determined. The major constituent fatty acids were: palmitic (37%) oleic (47%) and linoleic (8%). The major sterols were campesterol (42%) and stigmasterol (27%) and the major hydrocarbons were n‐C23 (37%), iso‐C29 (30%) and n‐C21 (19%). Minor constituents in each class of lipids were also detected and estimated. The oil produced was 54% on a dry weight basis. The fatty acid composition was found to be close to that of palm oil. Copyright © 1984 John Wiley &amp; Sons, Ltd

Journal	Phytochemistry
Publisher	-
ISSN	0031-9422