Pediocin production by Pediococcus acidilactici was studied in a bio-reactor under two fed batch fermentations (fed batch operation I and fed batch operation II) at constant pH condition using meat processing waste. In fed batch operation II, higher pediocin activity of 4572 AU/ml was achieved comparing to fed batch operation I (pediocin activity of 3657 AU/ml). The kinetics of cell growth was described by two substrates (glucose and protein) model. The correlation between two products (pediocin and lactic acid) and cell growth was described by Luedeking and Piret model. The comparison of simulated data with experimental results obtained from the study was well matched and the validated mathematical model was developed for fed batch processes. © 2020 Scientific Publishers. All rights reserved.