M GANGULY

K DATTA

A ROYCHOUDHURY

D GAYEN

D N SENGUPTA

S K DATTA

Fulltext

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Plant Signaling and Behavior

The present study evaluates the pattern of stress inducibility of one natural promoter (from rice Rab16A) and two synthetically designed promoters, viz., 4X ABRE (abscisic acid-responsive element, having four tandem repeats of ABRE) and 2X ABRC (abscisic acid-responsive complex, having two tandem repeats of ABRE and two copies of coupling elements), in response to varying concentrations of NaCl and abscisic acid (ABA). Each promoter, independently linked to gusA (that encodes β glucuronidase, GUS), was introduced into rice (cv. Khitish) through particle bombardment. The T2 progenies showed integration of gusA in their genome. The accumulation of gusA transcript, driven by each promoter in T2 transgenics, increased with increasing salt/ABA concentration, with ABA being the better activator of each promoter. Induction in GUS expression, driven by different promoters, was noted on exogenous salt/ABA treatments in a concentration-dependent manner. The maximum induction was observed with 2X ABRC promoter. All the three promoters could drive stress-inducible GUS expression in both vegetative and floral organs. However, prominent GUS expression was noted in the whole seed (both embryo and aleurone layer of endosperm) only by 2X ABRC, whereas it was localized only in the embryo for the other two promoters. Thus, our observation characterizes three efficient salinity/ABA-inducible promoters that have the potentiality in crop biotechnology to drive transgene expression for stress tolerance, whenever abiotic stress is encountered. © 2011 Springer-Verlag.

Plant Cell Reports

Inducibility of three salinity/abscisic acid-regulated promoters in transgenic rice with gusA reporter gene

In an attempt to understand the molecular basis of salt-stress response in the aromatic rice Gobindobhog, a comprehensive analysis encompassing physiological or biochemical assays and gene expression studies under high salt (200 mM NaCl) supply regimes were initiated and compared with a salt-sensitive (M-1-48) and salt-tolerant (Nonabokra) rice. The detrimental effects of salinity stress were the most pronounced in Gobindobhog, as reflected by the maximally increased root to shoot ratio, the highest chlorophyll degeneration, the highest foliar concentration of Na+ ions and peroxide content, with their maximum increment after salt treatment. The amplification of oxidative damages was further stimulated by the accumulation of putrescine and lipid peroxidation-derived toxic degradation products (increased malondialdehyde and lipoxygenase activity), which were comparable in M-1-48 and Gobindobhog. Antioxidants like anthocyanin and particularly cysteine and the osmolytes like reducing sugar, proline and polyamines (spermidine and spermine) showed the highest level in Nonabokra. While the inhibition of catalase activity occurred in all the varieties following salt-stress, the maximum induction in guaiacol peroxidase activity, elevated cysteine and proline levels in Gobindobhog probably constituted the detoxification mechanism obligatory for its survival. Intensification of the aroma content with salt treatment was markedly noted in Gobindobhog. A very low abundance of Rab16A/SamDC transcript and the corresponding proteins were observed both in M-1-48 and Gobindobhog, induced only after salt-stress, whereas they were constitutively expressed in Nonabokra. Thus, our data reflect Gobindobhog as a salt-sensitive cultivar, susceptible to high-stress-induced growth-inhibition, ion imbalances, membrane/oxidative damages with lower expression of stress-tolerant genes. © 2008 Springer-Verlag.

Comparative physiological and molecular responses of a common aromatic indica rice cultivar to high salinity with non-aromatic indica rice cultivars

Plant Biotechnology Journal

Comparative analysis of drought-responsive transcriptome in Indica rice genotypes with contrasting drought tolerance

Plant Molecular Biology

Inventory, evolution and expression profiling diversity of the LEA (late embryogenesis abundant) protein gene family in Arabidopsis thaliana

Journal of Agricultural and Food Chemistry

Correlation Analysis of Mineral Element Contents and Quality Traits in Milled Rice (Oryza stavia L.)

Overexpression of Rab16A gene in indica rice variety for generating enhanced salt tolerance

Journal	Plant Signaling and Behavior
Publisher	-
ISSN	1559-2316
Open Access	Yes