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Optimization of pediocin production by batch fermentation of pediococcus acidilactici NCIM 2292 using goat meat processing waste
, R. Chowdhury, C. Bhattacharjee
Published in
Volume: 8
Issue: 10
Pages: 19 - 25
Goat meat processing waste was enzymatically hydrolyzed using papain to utilize the soluble protein for the growth of Pediococcus acidilactici NCIM 2292 producing pediocin. The suitability of different carbohydrate, namely glucose, lactose, sucrose, maltose and fructose for biomass and bacteriocin production was also investigated. Glucose was observed to be the most preferred carbon source. The experiments based on five-level Central Composite Design (CCD) were conducted to study the effect of hydrolysis time of meat waste, initial pH and initial glucose concentration on the production of biomass and pediocin. The parameters were optimized by Response Surface Methodology (RSM). The optimum values of hydrolysis time, pH and glucose were determined to be 13 h, 6.5 and 20 g/l respectively. The results were analyzed statistically to evaluate analysis of variance (ANOVA). Under optimum condition, the biomass concentration and pediocin activity were measured to be 1.22 g/l and 2285 AU/ml at the end of the exponential growth phase (18 h). Antimicrobial activity of pediocin was assessed by well diffusion method using Staphylococcus aureus NCIM 2127 as indicator organism.
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JournalResearch Journal of Biotechnology