KISHOR SARKAR

Polymer Science and Technology

P P KUNDU

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Biopolymers: Biomedical and Environmental Applications

Fluorescent chitosan-graft-polyethyleneimine (PEI) copolymer was prepared by incorporating PEI molecule onto chitosan backbone through naphthalimide moiety by simple substitution reaction. 4-Bromo-1,8-naphthalic anhydride was used as fluorescent probe due to its strong fluorescence and good photo-stability property and the presence of a fine tunable bromide functional group in the naphthalimide ring, in this work. The copolymer was characterized by FTIR, elemental analysis and XRD. The fluorescence property of the copolymer was determined by UV-vis spectrometer and spectrofluorometer. The effects of pH and temperature on fluorescence property of the copolymer were also studied. The graft copolymer with degree of substitution 37.6 of PEI onto chitosan showed better complexation ability with DNA at comparatively low N/P (nitrogen to phosphate ratio) ratio 1.0 compared to that of chitosan (N/P ratio 2.0). The cytotoxicity of PEI largely decreased after grafting with chitosan and all the copolymers showed above 50% cell viability even at high polymer concentration (300 μg/mL). Therefore, the prepared fluorescent chitosan-graft-PEI copolymer may be used as a biological marker as well as drug or gene carrier with low toxicity. © 2012 Elsevier Ltd.

Carbohydrate Polymers

Preparation of low toxic fluorescent chitosan-graft-polyethyleneimine copolymer for gene carrier

Development of efficient and safe gene carrier is the main hurdle for successful gene therapy till date. Poor water solubility and low transfection efficiency of chitosan are the main drawbacks to be efficient gene carrier for successful gene therapy. In this work, PAMAM conjugated chitosan was prepared through naphthalimide moiety by simple substitution reaction. The synthesis of the chitosan conjugates was confirmed by FTIR, 1H NMR and XRD analyses. The conjugates showed enhanced DNA binding capability compared to that of unmodified chitosan. Moreover, the conjugates showed minimal cytotoxicity compared to that of polyethyleneimine (PEI, 25 kDa) and also showed good blood compatibility with negligible haemolysis. The transfection efficiency of the conjugate was significantly increased compared to that of unmodified chitosan and it also surpassed the transfection efficiency by PEI. Therefore, PAMAM conjugated chitosan can be used safely as alternate efficient gene delivery vector in gene therapy. © 2013 Elsevier Ltd. All rights reserved.

PAMAM conjugated chitosan through naphthalimide moiety for enhanced gene transfection efficiency

Proceedings of the National Academy of Sciences

PNAS Plus Significance Statements

The Obstetrician & Gynaecologist

Erratum

Biotechnology and Bioprocess Engineering

DNA/PEI/Alginate polyplex as an efficientin vivo gene delivery system

The Lancet

Safety and tolerability of gene therapy with an adeno-associated virus (AAV) borne GAD gene for Parkinson's disease: an open label, phase I trial

The AAPS Journal

In vitro evaluation of chitosan-EDTA conjugate polyplexes as a nanoparticulate gene delivery system

Natural Polymeric Vectors in Gene Therapy

Journal	Data powered by TypesetBiopolymers: Biomedical and Environmental Applications
Publisher	Data powered by TypesetJohn Wiley and Sons
Open Access	No