An efficient, rapid and large scale propagation of the elite exotic, fruit yielding, medicinal plant, Cyphomandra betacea (Family Solanaceae) through in vitro axillary bud multiplication, is described. Murashige and Skoog's medium supplemented with 5mgl-1 6-BAP was the most effective in inducing bud break and growth, and also in initiating multiple shoot proliferation at the rate of 96 microshoots per axillary bud after 30-35 days of culture. By repeated subculturing on the primary inductive media, a high frequency multiplication rate was established. Efficient rooting was achieved with 1.5mgl-1 Indole butyric acid (IBA). The higher percentage of root initiation (88%) was noted in this concentration of IBA. Rooted microshoots were passed through acclimatization phases prior to ex vitro transfer. Micropropagated plants established in soil were morphologically uniform and identical to donor plants.