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Metabolomic and chemometric study of Achras sapota L. fruit extracts for identification of metabolites contributing to the inhibition of alpha-amylase and alpha-glucosidase
Published in SPRINGER
Volume: 242
Issue: 5
Pages: 733 - 743
Achras sapota L. fruit extracts have previously been reported to have activities against the carbohydrate-digesting enzymes alpha-amylase and alpha-glucosidase. The present work was aimed to identify the inhibitors of alpha-amylase and alpha-glucosidase using metabolomics and chemometric approaches. Metabolite profile of Achras sapota fruit extracts at different stages of post-harvest ripening was analyzed using GC-MS-based metabolomics approach. The extracts were also assayed for their properties to inhibit the carbohydrate-digesting enzymes alpha-amylase and alpha-glucosidase in vitro. The fruit extracts just after harvesting showed maximum activities against the enzymes alpha-amylase and alpha-glucosidase. The activities declined with post-harvest ripening. It was observed from the chemometric analysis that gallic acid, chlorogenic acid, benzene-1,2,4-triol, and d-malic acid are responsible for high alpha-amylase inhibitory activity. Gallic acid and chlorogenic acid are positively correlated with high alpha-glucosidase inhibitory activity. Inhibition of the two enzymes by gallic acid, benzene-1,2,4-triol, chlorogenic acid, and alpha-amylase by d-malic acid supported the findings. The present experiment reports the importance of both primary and secondary metabolites for the bioactivity of plant extracts.
About the journal
JournalData powered by TypesetEuropean Food Research and Technology
PublisherData powered by TypesetSPRINGER