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Marrying chemistry with biology by combining on-chip solution-based combinatorial synthesis and cellular screening
M BENZ, , A BöSER, A ROSENFELD, P A LEVKIN
Published in Nature Publishing Group
2019
Volume: 10
   
Issue: 1
Abstract
Drug development often relies on high-throughput cell-based screening of large compound libraries. However, the lack of miniaturized and parallelized methodologies in chemistry as well as strict separation and incompatibility of the synthesis of bioactive compounds from their biological screenings makes this process expensive and inefficient. Here, we demonstrate an on-chip platform that combines solution-based synthesis of compound libraries with high-throughput biological screenings (chemBIOS). The chemBIOS platform is compatible with both organic solvents required for the synthesis and aqueous solutions necessary for biological screenings. We use the chemBIOS platform to perform 75 parallel, three-component reactions to synthesize a library of lipidoids, followed by characterization via MALDI-MS, on-chip formation of lipoplexes, and on-chip cell screening. The entire process from the library synthesis to cell screening takes only 3 days and about 1 mL of total solutions, demonstrating the potential of the chemBIOS technology to increase efficiency and accelerate screenings and drug development. © 2019, The Author(s).
About the journal
JournalNature Communications
PublisherNature Publishing Group
ISSN2041-1723
Open AccessYes