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Low molecular weight polyethyleneimine conjugated guar gum for targeted gene delivery to triple negative breast cancer
P. Jana, S. Ghosh,
Published in Elsevier B.V.
PMID: 32553957
Volume: 161
Pages: 1149 - 1160
The study emphasized on the development of an efficient, receptor-targeted non-viral gene delivery vehicle for gene therapy of triple negative breast cancer (TNBC). Here, naturally abundant guar gum based non-viral carrier was developed through conjugating by low molecular weight polyethylenimine (LPEI) (GNP) using napthalic anhydride coupling agent and characterized them by FT-IR, 1H NMR, XRD and UV spectrophotometer. The carrier was found to be cytocompatible as revealed by MTT assay against MDA-MB-231 and HeLa cell lines and excellent blood compatibility till the concentration of 200 μg/ml. In addition to these, the carrier exhibited excellent gene binding capability and formed spherical shaped complexes. The carrier showed very high in vitro transfection efficiency in TNBC cell (MDA-MB-231) compared to lipofectamine 2000 (LF2k) which could be justified by its high buffering capacity. Therefore, GNP may be an attractive non-viral gene carrier for gene therapy of TNBC in future. © 2020 Elsevier B.V.
About the journal
JournalData powered by TypesetInternational Journal of Biological Macromolecules
PublisherData powered by TypesetElsevier B.V.