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Lipolytic enzymes of trichophyton rubrum
S K DAS, A B BANERJEE
Published in Informa Healthcare
1977
Volume: 15
   
Issue: 3
Pages: 313 - 323
Abstract
Trichophyton rubrum cells contain lipase, phospholipases A and B and acyl CoA lysolecithin acyl transferase activities. This dermatophyte excretes lipase and phospholipase A into the growth medium when cultivated in Sabouraud's broth. Extracellular lipase has optimum activity at pH 8.0 whereas the intracellular lipase is maximally active at pH 8.0 whereas the intracellular lipase is maximally active at pH 7.0. The optimum pH of phospholipase A and B activities which are localized in 15000 g sedimentable cell fragments are 7.0 and 6.0 respectively. Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity. © 1977 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
About the journal
JournalMedical Mycology
PublisherInforma Healthcare
ISSN1369-3786