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Karyotype stability in long-term callus derived plants of Crepis tectorum L.
J SENGUPTA, S JHA, S SEN
Published in Springer Netherlands
1988
Volume: 30
   
Issue: 4
Pages: 247 - 251
Abstract
The study of in vitro growth of Crepis tectorum revealed 100 % callusing and 40 % plantlet regeneration. The root and leaf used as explants showed the normal diploid (2n=8) chromosome constitution. In one month old culture 95 % callus cells were diploid. The callus maintained in 2,4-D 1 mg 1-1 for two years showed 62 % diploid, 5 % tetraploid and 33 % hyperdiploid cells. The differentiation of shoot occurred in two year old calli after subeulturing in 2 mg I-1 BAP and the potentiality of regeneration was retained for more than one year. The leaf-tips of regenerated plants were homogeneous and identical to the donor plant both in number and morphology of chromosomes. © 1988 Academia.
About the journal
JournalData powered by TypesetBiologia Plantarum
PublisherData powered by TypesetSpringer Netherlands
ISSN0006-3134