Chromosome characters of three indigenous medicinal plants of high repute were studied. Their karyotypes were commonly mono-modal, decreasing in length from the longest to the shortest chromosomes. The chromosome complement in centromeric positions was 6sm+34M+24m for Bacopa monnieri (L.)Wettst. (2n064), 2m.st+12M+6sm+2m for Tylophora indica (Burm.f.) Merrill (2n022) and 2m.st+4m.sm+4M+18m+20sm for Withania somnifera (L.) Dunal (2n048). © Archana Sharma Foundation of Calcutta 2012.