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Isolation and characterization of nickel-resistant microflora from serpentine soils of Andaman
A PAL, P CHOUDHURI, S DUTTA, P K MUKHERJEE, A K PAUL
Published in SPRINGER
2004
Volume: 20
   
Issue: 9
Pages: 881 - 886
Abstract
Serpentine soils of Andaman Islands, India characteristically contain high levels of nickel, cobalt and chromium and are colonized by indigenous nickel-hyperaccumulating plants. Attempts have been made to isolate and characterize nickel-resistant microorganisms from these hitherto unexplored naturally nickel-percolated soils. The majority of the nickel-resistant organisms showed a minimum inhibitory concentration (MIC) of Ni2+ ranging from 300 to 400 mg/l and about 3.4% of the total 89 isolates representing bacterial strains were able to grow at 400 mg/l Ni2+. The potent Ni2+-resistant strains AND305 and AND603 were tentatively identified as Pseudomonas spp. and strain AND408 as Bacillus sp. following detailed analysis of morphological and physio-biochemical characteristics. Growth kinetics of these Ni2+-resistant bacteria showed a prolonged lag phase in Ni2+-containing media, which extended with increasing nickel concentration. In addition to Ni2+, these isolates were also resistant to Co2+, Cd2+, Cr6+, Fe3+, Cu2+, Mg2+, Mn2+ (50-200 mg/l) and Hg 2+ (0.5-2.0 mg/l) and the multiple metal-resistance of the isolates were also associated with the resistance to antibiotics ampicillin, cycloserine and penicillin G.
About the journal
JournalData powered by TypesetWorld Journal of Microbiology and Biotechnology
PublisherData powered by TypesetSPRINGER
ISSN0959-3993