Two fungi, responsible for the degradation of starch/polyvinyl alcohol (PVA) biocomposite films were isolated from aerobic compost environment by extensive screening. ITS (Internal Transcribed Spacer) sequencing of the two fungi samples showed 99% identity at 99% coverage with the sequence from Penicillium daleae, and 100% identity at 100% coverage with the sequence from Aspergillus flavus. An extensive weight loss study was performed using these two fungi on starch/PVA films separately. In comparison to the weight loss observed in the compost (nearly 60%), starch/PVA films exhibited a higher weight loss (nearly 71%) by these two fungi after 30 days of degradation. The borax crosslinked films exhibited 85% degradation by A.flavus. After 15 days of degradation by fungus closely related to P.daleae, the borax crosslinked films disappeared completely. This study showed that the A.flavus and fungus with close affinity to P.daleae can degrade such biocomposite films more efficiently in comparison to biodegradation in compost. © 2013 Elsevier Ltd.