Objectives: The present study is aimed at investigating the involvement of oxidative stress in ischemic heart disease (IHD) of patients in tertiary care hospital in Kolkata.Methods: Serum parameters of myocardial injury, oxidative stress biomarkers and antioxidant enzymes were measured. These data were supported by the data of electrocardiography and echocardiography. Results: The current studies indicate involvement of oxidative stress in Indian IHD patients, and assessing these biomarkers may be useful in diagnosis of patients with ischemic hypertrophic cardiomyopathy.Conclusion: Cardiovascular diseases are becoming one of the leading health problems in India. The majority of cardiovascular diseases and related complications occur due to imbalance of oxidants and pro-oxidants in an individual. Thus, oxidative stress is one of the main factors in ischemic heart disease (IHD).