Header menu link for other important links
X
Induction of micronuclei by zinc in human leukocytes: A study using cytokinesis-block micronucleus assay
S K DAS, G TALUKDER, A SHARMA
Published in -
2002
Volume: 88
   
Issue: 2
Pages: 139 - 144
Abstract
In the present study, we report the results of the capability of zinc chloride for the induction of micronuclei in cultured human leukocytes using cytokinesis-block micronucleus assay. Two concentrations of zinc chloride (1.5 × 10-4 M and 3.0 × 10-4 M) were used to evaluate the potential of this zinc salt to induce micronucleus formation. This effect was compared with positive (mitomycin C treated) and negative controls (no salt added). Our results show a significant (p ≤ 0.001) increase of micronucleated cytokinesis-blocked cells (MNCBs) in zinc-chloride-treated cells compared to the negative control. Induction of MNCBs was not in a dose-dependent manner for zinc chloride concentrations tested. This report is the first to describe the efficiency of cytokinesis-block micronucleus assay to evaluate the genotoxic effects of zinc salt.
About the journal
JournalBiological Trace Element Research
Publisher-
ISSN0163-4984