In the present study, we report the results of the capability of zinc chloride for the induction of micronuclei in cultured human leukocytes using cytokinesis-block micronucleus assay. Two concentrations of zinc chloride (1.5 × 10-4 M and 3.0 × 10-4 M) were used to evaluate the potential of this zinc salt to induce micronucleus formation. This effect was compared with positive (mitomycin C treated) and negative controls (no salt added). Our results show a significant (p ≤ 0.001) increase of micronucleated cytokinesis-blocked cells (MNCBs) in zinc-chloride-treated cells compared to the negative control. Induction of MNCBs was not in a dose-dependent manner for zinc chloride concentrations tested. This report is the first to describe the efficiency of cytokinesis-block micronucleus assay to evaluate the genotoxic effects of zinc salt.

S K DAS

G TALUKDER

A SHARMA

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Induction of micronuclei by zinc in human leukocytes: a study using cytokinesis-block micronucleus assay.

Biological Trace Element Research

The degree of chromosome damage induced by three compounds of zinc (zinc chloride, zinc sulfate, zinc acetate) was compared in human leucocytes in vitro. Three concentrations of each salt, 3.0 × 10-5M, 3.0 × 10-4M, and 1.5 × 10-3M, were added to leukocyte cultures. The cells were harvested after 48 and 72 h and chromosome spreads were prepared following a colchicine-hypotonic-fixation-air drying-Giemsa staining schedule. The end point screened was chromosome aberrations. All three salts were lethal at the highest concentration. The degree of chromosome damage was directly proportional to the concentrations used for zinc sulfate and zinc acetate but not for zinc chloride.

Comparison of chromosome damage induced by three zinc compounds using human leukocyte culture

Nutrition Reviews

ACRODERMATITIS ENTEROPATHICA-HEREDITARY ZINC DEFICIENCY

Mutation Research/Reviews in Mutation Research

Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project

Science

Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

The in vitro micronucleus technique

Induction of micronuclei by zinc in human leukocytes: A study using cytokinesis-block micronucleus assay

Journal	Biological Trace Element Research
Publisher	-
ISSN	0163-4984