Response to in vitro culture of Scilla siberica was found to be both explant and hormone specific. Bulb scale explant showed optimum response to callusing in MS medium with 2,4-D and kinetin supplement. Leaf explants did not show callusing and optimum result for direct regeneration of shoots was obtained on MS medium with BAP supplement. This explant specificity to in vitro response in S. siberica is evidently under genetic control and a measure of genetic diversity. Chromosome studies of regenerants also indicate the presence of marker heteromorphic chromosomes as reported in in vitro plants in this laboratory. The aged callus also retains cell viability but regeneration competence of the variants was not recorded in the medium used. © 2002 Elsevier Science B.V. All rights reserved.

D CHAUDHURI

S SEN

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

In vitro response of Scilla siberica

Scientia Horticulturae

Callus cultures were raised from bulb scale segments of Orinthogalum umbellatum L. (Liliaceae), on a Murashige and Skoog (1962) medium (MS) with 8 mg/l[Figure not available: see fulltext.] naphthaleneacetic acid (NAA). Bulbous shoots developed from calli after 2 months using MS medium with 2 mg/l NAA and 0.5 mg/l N6 - benzyladenine (BA). Shoots were also induced directly from scales of regenerated bulb used as secondary explants on MS medium supplemented with 0.5 mg/l BA. Shoots developed roots in 1/2 - strength MS medium. Regenerants multiplied rapidly in 1/2-MS liquid medium. Chromosome instability was reduced in callus grown on 2 mg/l NAA compared to callus grown on 8 mg/l NAA. Callus retained regeneration potential for 5 years in this modified MS medium. The chromosome analysis of regenerants dervied from callus, even from long term culture of 5 years, revealed only diploid cells with normal karyotype comprising 2n=46 chromosomes. Stable nature of callus and regenerants were further confirmed by cytophotometry. This procedure can be applied for securing stable regenerants on a mass scale in O. umbellatum. © 1995 Springer-Verlag.

Plant Cell Reports

Rapid and stable propagation of Ornithogalum umbellatum L. in long term culture

For in vitro regeneration spear, shoot tip, internode, node and root of mature plants of Asparagus cooperi were used as explants. Induction of callus from different explants depended on the photoperiod in addition to a specific combination of α-napthalene acetic acid (NAA) and kinetin (Kn) in the basal medium. The development of shoots from callus required 6-benzylaminopurine (BA), l-arginine, adenine and a low level of NAA. The individual shoots produced roots in the presence of indole-3-butyric acid (IBA) or indole-3-butyric acid containing potassium salt (KIBA). Regenerated plants were cytologically and phenotypically stable. © 1992.

Plant Science

Stable regeneration in Asparagus cooperi Baker as controlled by different factors

Somatic embryogenesis and plantlet formation were obtained from callus derived from the subapical region of spears of Asparagus cooperi Baker. Callus was obtained in Murashige and Skoog's medium supplemented with 1-naphthaleneacetic acid and kinetin. Increase in the concentration of potassium nitrate in subsequent subcultures resulted in the formation of embryos. Rapid multiplication of embryos was secured on transfer to a medium containing a different source of nitrogen and a low level (0.01 mg/1) of gibberellic acid. Media containing zeatin or gibberellic acid led to the formation of complete plantlets from embryos. Regenerated plants were cytologically and phenotypically stable. © 1991 Springer-Verlag.

Plant regeneration through somatic embryogenesis from spear callus culture of Asparagus cooperi Baker

Plants were regenerated from callus derived from stem explants of Ruscus hypophyllum L. The regenerating callus could be maintained by subculturing to basal medium containing BAP (0.5 mg/l) for over five years. Detailed analysis of mitotic and meiotic chromosomes and phenotypes of regenerants for a period of three years revealed no significant differences between cultured and non-cultured plants. The preliminary evidences indicate that genetic stability is maintained in this species generated from compact callus which provides with a source of rapid multiplication. © 1989, Japan Mendel Society, International Society of Cytology. All rights reserved.

Fulltext

CYTOLOGIA

Stable regenerants from long-term callus cultures of ruscus hypophyllum L

Embryogenic calli were formed when one year old friable calli, obtained from bulb scale explants, were allowed to remain on the high 2,4-D medium for a prolonged period. Globular embryoids were induced to develop into complete bulbous plants with following sequential transfer to MS medium containing 0.05 − 0.1 mg ·1−1 BAP (or 15 % CM or BAP 0.1 mg ·1−1 + CM 10 %) for 4 – 6 weeks and then to MS medium + 0.01 mg · 1−1 NAA + 0.05 mg · 1−1 Kn for 8 weeks. A final period in liquid MS medium stimulated shoot and root growth to the point where successful transplanting of plants to soil was assured. © 1986, Gustav Fischer Verlag, Stuttgart. All rights reserved.

Journal	Data powered by TypesetScientia Horticulturae
Publisher	Data powered by TypesetELSEVIER SCIENCE BV
ISSN	0304-4238