Multiple shoot formation and their elongation from excised apical vegetative shoots of a 40-year old-tree of Mitragyna parvifolia Korth. was achieved in Murashige and Skoog's medium supplemented with 4.44 μM benzyl adenine. The in vitro regenerated shoots rooted when cultured on modified Murashige and Skoog's medium containing low inorganic salts and the three auxins. Regeneration by this method was suitable for mass propagation of the plant. © 1988 Martinus Nijhoff Publishers.