Upon attacked by pathogens, plants defend themselves by producing array of defense related molecules. Plant peroxidase enzymes are about 300-350 residue long and have multiple isoenzymes that differ in substrate specificity and localization within the plant. In this study, 3D structure of peroxidase enzymes from five different plants was predicted by homology modeling method. The quality of the 3D structure of the model was confirmed by various web based validation programs. When compared secondary and tertiary structure of the model, it showed two peroxidase signature domains (PEROXIDASE_1 and PEROXIDASE_2) are present in the central region. Degree of sequence conserveness showed that sequence size differences do not make any impact on their basic functions.