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Improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles
A MUKHOPADHYAY, A K DASGUPTA, D CHATTOPADHYAY, K CHAKRABARTI
Published in ELSEVIER SCI LTD
2012
Volume: 116
   
Pages: 348 - 354
Abstract
The activity and half-life of pectate lyase (PL) from Bacillus megaterium were nine- and 60-fold, respectively, higher at 90°C in the presence of hydroxyapatite nanoparticles (NP-PLs) than in the presence of 1mM CaCl 2. Thermodynamic analysis of the nanoparticle-induced stability revealed an enhanced entropy-enthalpy compensation by the NP-PLs since a reciprocal linearity of the enthalpy-entropy change to 90°C was observed. Without nanoparticles, the linearity range was 70°C. Such compensation reflected the maintenance of the native structure of proteins. The remarkable enhancement of activity and stability of the NP-PL system at high temperatures may be utilized commercially e.g. in the food industry or the processing of natural fibers that may require a thermotolerant enzyme. © 2012 Elsevier Ltd.
About the journal
JournalData powered by TypesetBioresource Technology
PublisherData powered by TypesetELSEVIER SCI LTD
ISSN0960-8524