Chronic hepatotoxicity was induced by po introduction of CCl4 (2.5 ml/kg body weight/day) in paraffin oil. Administration of ethanolic extract of Astraeus hygrometricus (150 mg/kg body weight/day) orally protected the CCl4 mediated elevation of serum transaminase such as glutamate pyruvate transaminase (GPT) and glutamate oxaloacetate tansaminase (GOT) and of serum alkaline phosphatase (ALP), total bilirubin, and direct bilirubin. The hepatic antioxidant status such as superoxide dismutase and catalase activities were reduced in the CCl4 alone treated animals. Administration of extract to CCl4 challenge restored the hepatic antioxidant status. The findings thus suggested ethanolic extract of A. hygrometricus protects CCl4 induced chronic hepatotoxicity in mice by restoring the liver antioxidant status.