A suspension cell culture was initiated from callus cells originating from Arachis hypogea cotyledon explants in the presence of 1-napthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). Exogenous NAA depletion did not affect the growth of these cells, indicating them to be auxin-autotrophic in nature. Unlike the NAA-supplemented counterparts, the NAA-depleted cells were unable to grow after being exposed to stress conditions that led to the loss of water. The rate and amount of water loss from both the NAA-supplemented and the NAA-deprived cells were found to be the same, indicating that the presence of NAA did not enable stress avoidance. Our results suggest that NAA contributed tolerance to these cells by allowing them to recover after water loss.

ANINDITA SEAL

Biotechnology and Dr. B. C Guha Centre for Genetic Engineering and Biotechnology

Maitrayee DasGupta

Bio-Chemistry

A HAZRA

R NAG

S CHAUDHURI

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Plant Cell Reports

Arachis hypogea is a non-"infection thread" (IT) legume where rhizobial entry or dissemination in the nodules never involves IT. Rhizobia invade through epidermal "cracks" and directly access the cortical cells to develop the characteristic aeschynomenoid nodules. For investigating these nonclassical nodulation features in Arachis spp., we developed an efficient procedure for Agrobacterium rhizogenes R1000-mediated transformation of this plant. In this study, we optimized the induction of hairy roots and nodulation of composite Arachis hypogea plants in the presence of Bradyrhizobium sp. (Arachis) strain NC92. 35S promoterdriven green fluorescent protein and β-glucuronidase expression indicated transformation frequency to be above 80%. The transformed roots had the characteristic rosettetype root hairs and had normal level of expression of symbiosis-related genes SymRK and CCaMK. The transgenic nodules resembled the wild-type nodules with an exception of 2 to 3%, where they structurally deviated from the wildtype nodules to form nodular roots. A 16S rRNA profile of an infected-zone metagenome indicated that identical populations of bradyrhizobia invaded both composite wild-type plants grown in natural soil. Our results demonstrate that Arachis hairy root is an attractive system for undertaking investigations of the nonclassical features associated with its nitrogen-fixing symbiotic interactions. © 2009 The American Phytopathological Society.

Fulltext

Molecular Plant-Microbe Interactions

Transformed hairy roots of Arachis hypogea: A tool for studying root nodule symbiosis in a non-infection thread legume of the aeschynomeneae tribe

An auxin autotrophic Arachis hypogea cell culture was sensitive to stress treatments leading to water loss whereas the growth of its auxin-supplemented counterpart was unaffected under similar conditions. Here we show that an hour of transient auxin treatment in the post stress period was sufficient for restoring the auxin autotrophic growth potential of the stress driven quiescent Arachis cells. Qualitative proteome analysis revealed protein turnover to have a role in mediating auxin-originated signals in these cells. In consonance, MG132 a cell permeable inhibitor of the ubiquitin mediated protein turnover completely inhibited the auxin dependent growth restoration of the stressed Arachis cells. Thus protein turnover is a necessary downstream event in exogenous auxin mediated stress tolerance in Arachis cells. © Springer 2005.

Plant Cell, Tissue and Organ Culture

Protein turnover in response to transient exposure to exogenous auxin is necessary for restoring auxin autotrophy in a stressed Arachis hypogea cell culture

The ABA responsive ABI3 and the auxin responsive ARF family of transcription factors bind the CATGCATG (Sph) and TGTCTC core motifs in ABA and auxin response elements (ABRE and AuxRE), respectively. Several evidences indicate ABI3s to act downstream to auxin too. Because DNA binding domain of ABI3s shows significant overlap with ARFs we enquired whether auxin responsiveness through ABI3s could be mediated by their binding to canonical AuxREs. Investigations were undertaken through in vitro gel mobility shift assays (GMSA) using the DNA binding domain B3 of PvAlf (Phaseolus vulgaris ABI3 like factor) and upstream regions of auxin responsive gene GH3 (-267 to -141) and ABA responsive gene Em (-316 to -146) harboring AuxRE and ABRE, respectively. We demonstrate that B3 domain of PvAlf could bind AuxRE only when B3 was associated with its flanking domain B2 (B2B3). Such strict requirement of B2 domain was not observed with ABRE, where B3 could bind with or without being associated with B2. This dual specificity in DNA binding of ABI3s was also demonstrated with nuclear extracts of cultured cells of Arachis hypogea. Supershift analysis of ABRE and AuxRE bound nuclear proteins with antibodies raised against B2B3 domains of PvAlf revealed that ABI3 associated complexes were detectable in association with both cis elements. Competition GMSA confirmed the same complexes to bind ABRE and AuxRE. This dual specificity of ABI3 like factors in DNA binding targeted to natural promoters responsive to ABA and auxin suggests them to have a potential role in conferring crosstalk between these two phytohormones. © Springer 2005.

Journal	Data powered by TypesetPlant Cell Reports
Publisher	Data powered by TypesetSPRINGER
ISSN	0721-7714
Open Access	No