Solanum nigrum, Phyllanthus amarus and Heliotropium indicum are three medicinal plants indigenous to Asia belonging to the family Euphorbiaceae, Solanaceae and Boraginaceae respectively. They have been traditionally used in ancient medicine for treating variety of diseases. In our present study, the extract fractions (aqueous, chloroform and hexane) of these three plants have been tested against three cervical cancer cell lines – HeLa (HPV 18 positive), SiHa (HPV 16 positive) and C33A (HPV negative). The antiproliferative activity of plant extracts were evaluated by standard cytotoxicity measuring assay like 3-(4, 5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) reduction assay. Genotoxicity was measured by Comet assay or Single Cell Gel Electrophoresis (SCGE) assay. Plant extract fractions showed varied levels of cytotoxicity which depends on cell types and also on the nature of extract fractions. The IC50 doses determined using MTT assay was found to be non-toxic to normal mouse embryonic cell line NIH3T3. However the fractions which showed anti-proliferative activity at low dose level were considered effective. Among the medicinal plant extract chloroform fraction of Solanum nigrum, Phyllanthus amarus and both chloroform and hexane fraction of Heliotropium indicum were found to be most efficient in inhibiting cell growth and inducing apopotosis. © 2015 Global Research Online. All rights reserved.