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Enzymatic reactions on thin-layer chromatographic plates. IV. Lipolysis of wax esters and separation of products on a single plate
S MISRA, A CHOUDHURY, A KR DUTTA, A GHOSH, J DUTTA
Published in -
1983
Volume: 280
   
Pages: 313 - 320
Abstract
Hydrolysis of wax esters on thin-layer chromatographic plates by porcine pancreatic lipase is described. The alcohols and free fatty acids produced were separated on the same plate, recovered and then analysed by gas-liquid chromatography for their compositions. Synthetic wax containing saturated fatty acids of various chain lengths and unsaturated fatty acids with varying numbers of methylene-interrupted cis double bonds, along with beeswax and Avicennia officinalis leaf wax were lipolysed. The results of on-plate lipolysis for 96 min compared well with those obtained by chemical hydrolysis in tubes. © 1983.
About the journal
JournalJournal of Chromatography A
Publisher-
ISSN0021-9673