Digital microfluidic biochips are restructuring many areas of Biochemistry, Biomedical sciences, and Microelectronics. It is also known as 'Lab-on-a-Chip' for its recognition as a substitute for laboratory experiments. In recent times, due to emergency and cost efficacy, more than one assay operations are required to be performed at the same time. So, parallelism is a must in designing biochips. Having an area of a given chip as a constraint, how efficiently we can use a restricted sized chip and how much parallelism can be built-in are the objectives of this paper. A specific application of an assay may characterize a sample where, say only one type of reagent and multiple samples have been considered, or vice versa, and identify some parameter(s) of the sample(s) under requirement in parallel. In our experimentation, we essentially do this task in parallel for five such sets of subregions of a given restricted sized chip in digital microfluidics using an array based partitioning pin assignment technique, where cross contamination problem has also been considered, and efficiency of proper taxonomy of a given sample has also been improved. © 2014 IEEE.