Microfluidic biochip is a lab-on-a-chip system that replaces conventional laboratory experiments. Digital Microfluidic Biochip (DMFB) handles liquids as discrete droplets, and offers highly reconfigurable and scalable technology. DMFB combines electronics with biology opening the new application areas of Microelectronics, Biochemistry, and Biomedical sciences. A new application area of DMFB using equilateral triangular electrodes instead of square electrodes has been proposed, considering the design issues and the fluidic constraints while performing all the modular operations. The improvement of sample-reagent mixing procedure is a key challenge issue in bioassay implementation as mixing is the most dominating operation in DMFB; hence, the Triangular DMFB (TEDMB) system leads over the existing DMFB system. In this paper, we have presented a study of TEDMB mixers and developed mixing library for TEDMB synthesis. © 2017 IEEE.