Casein hydrolysate at 2.0 g l-1 significantly enhanced forskolin content (2.3 mg g-1 cell dry wt) in a rhizogenic tumourous line, GCO-RCH-2 of Coleus forskohlii. In rooty teratoma line, RC-ST-2/4, forskolin content enhanced to 1.7 mg g-1 cell dry wt in presence of 2.5 g l-1 casein hydrolysate. Unlike untransformed calli and rhizogenic/root cultures, all the forskolin yielding transformed cultures of C. forskohlii have been maintained for over 5 years.

S MUKHERJEE

B GHOSH

S JHA

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Enhanced forskolin production in genetically transformed cultures of Coleus forskohlii by casein hydrolysate and studies on growth and organisation

Biotechnology Letters

Increased forskolin yield was obtained in transformed root, rhizogenic calli and cell suspension cultures of Coleus forskohlii when treated alone with various concentrations of auxins (IAA, IBA, NAA, 2,4-D), auxin conjugates (IAA-ala, IAA-gly, IAA-phe, IAA-asp), cytokinins (Kn, BAP) and gibberellic acid (GA3), An 8.9-fold stimulation in forskolin production was achieved in transformed rhizogenic line GCO-RCH-10 in presence of 1.0 mg I-1 GA3, 6-fold in cell suspension line GSO-5/7-k in presence of 2 mg I-1 BAP and 4.3-fold in root line RC-ST-2/16 in presence of 0.5 mg I-1 GA3 at the end of a culture period of 4 weeks. Growth and morphology was found to be influenced by the growth regulators studied. A seven fold increase in biomass was obtained in rhizogenic line GCO-RCH-10 with 0.5 mg I-1 GA3. In root line RC-ST-2/16, different concentrations of IAA, IAA conjugates and GA3 stimulated growth while cytokinins inhibited growth of roots. The shoot culture line ST-2/51/d, showed prolific growth in the presence of all cytokinins but no forskolin was detected in the shoot cultures treated with any of these hormones.

Journal	Data powered by TypesetBiotechnology Letters
Publisher	Data powered by TypesetSPRINGER
ISSN	0141-5492