S MUKHOPADHYAY

D K BHATTACHARYA

S ADDYA

G C CHATTERJEE

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Letter to the editor: Effects of mercuric chloride treatment in vitro on the antioxidant defence system and activation of human blood platelets

International Journal of Environmental Studies

Aim The torrential hill streams of eastern Himalayan region represent a unique lotic water ecosystem. The fish population inhabiting this regionaredesignated as habitat specialists. Being good indicator of different environmental factors, haematopoiesis can be considered as an important component in survival strategy of such characteristic fish. So, the aim of the present study was to perform quantitative and qualitative evaluation of developing blood cells in the head kidney of two such habitat specialist fish species of River Mu rti- Garra gotyla gotyla and Garra annandalei. Methodology Garra gotyla gotyla and Garra annandalei we re collected from three different sites of river Murti selected along decreasing altitudinal gradient and different habitat type. Tissue imprint technique and haematopoietic efficiency determination method we re employed for qualitative and quantitative analysis of haematopoietic cells. Cell cycle phase distribution of nuclear DN Aof head kidney cells were determined by flow cytometry. Results Eleven types of hematopoietic precursors and mature cells in erythroid and lymphoid lineage at different developmental stages were identified and documented through cytological description and morphometry in head kidneys of both the species. The erythroid lineage was dominant over all other lineages with relatively less storage forms and more young and mature eiythrocyte population which was about 55.73% and 47.74% of the whole haematopoietic components studied in Gaira gotyla gotyla and Garra annandalei, respectively. The flow cytometry analysis of the head kidney cells of both the species according to their Forward scatter (size) and Side scatter (granularity) properties revealed two distinct populations corresponding to small agranular and large granular cells. The percentage distribution of such cells by flow cytometry corroborated with the observation under light microscopy. Cell cycle analysis and erythropoietic efficiency of head kidney also revealed significantly high maturation rate of developing blood cells in Garra gotyla gotyla than Garra annandalei. Interpretation The differential pattern of erythropoiesis might indicate its functional significance reflecting differences in the survival strategies and habitat selection of Garra gotyla gotyla and Garra annandalei.

Fulltext

Journal of Environmental Biology

Understanding haematopoiesis as survival strategy in two characteristic habitat specialist fishes inhabiting a hill stream environment

Interactions of human platelets with cadmium in vitro were studied with respect to the platelet activation process as indicated by malondialdehyde (MDA) formation and also to the components of the cellular antioxidant defence system such as catalase, glutathione peroxidase (GP), glutathione reductase (GR), glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PDH), and reduced glutathione (GSH). Cadmium treatment stimulated platelet MDA formation after a lag phase of at least 15 min and this effect was completely blocked by either 1 mM aspirin or 1 mM CaC12. Cadmium pretreated platelets also displayed a much higher (5 fold) MDA formation when stimulated by thrombin. Platelet catalase activity was decreased by almost 50% after incubation with cadmium. There was also a moderate decline in platelet GSH and GR activity along with a stimulation of GST and G6PDH activity. These results suggest : (1) the cadmium effect on platelets as observed by enhanced formation of MDA via the cyclooxygenase pathway involves intraplatelet accumulation of cadmium which is inhibited by calcium, (2) a modest decline in GSH, presumably due to the inadequacy of H202 detoxification mechanism, does not adversely affect platelet function because of the adaptive response of G6PDH, and (3) intracellular accumulation of cadmium may result in platelet hyperactivity through higher intraplatelet free calcium levels resulting directly through cadmium action or indirectly through higher H202 levels due to catalase inhibition. © 1988.

Thrombosis Research

Effects of cadmium treatment invitro on the antioxidant protection mechanism and activation of human blood platelets

Atherosclerosis

Effect of sulfhydryl inhibitors and membrane-active drugs on platelet reactions

Experimental Biology and Medicine

Inhibition of Platelet Adhesion to Collagen by Sulfhydryl Inhibitors

Effects of mercuric chloride treatment in vitro on the antioxidant defence system and activation of human blood platelets

Journal	International Journal of Environmental Studies
Publisher	-
ISSN	0020-7233