Bioactive glass 46.1 SiO2 24.4 Na2O (26.9-x) CaO 2.6 P2O5.xSrO (x = 0, 2, 4, 6 mol%) have been prepared using the conventional melting and annealing method. The glass samples were characterized by Density measurement, Powder X-Ray Diffraction (XRD), Fourier Transformed Infrared (FTIR) spectroscopy and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDX). Bioactivity of the glass was measured by SEM with EDS, pH measurement and ionic concentrations analysis after immersion in SBF solution for different days at 37.59 degrees C. Experimental results showed that SrO substitution for CaO in the glass increased glass density and molar volume whereas oxygen density was decreased. The glass transition temperature and crystallization temperature were decreased with increase in strontium oxide substitution. The XRD and FTIR spectra showed the formation of the crypto crystalline hydroxy apatite (HA) on the surface of bioactive glass samples after immersing in SBF for 14 days. SEM of the 14 days immersed sample shows the formation of cotton ball like HCA layer on the glass. EDS analysis also confirmed the formation of HCA layer. Effect of strontium oxide substitution on the pH, silicon ion concentration and the weight loss after SBF immersion of the different glass samples are discussed.