To overcome the limitation of low productivity, some conditions arc to be established for the control of micro organism activity, so that their multiplication and successive functioning can be favored. During the growth period, mineral supplements are necessary to enhance the activity of different enzymes. Addition of right mixture of minerals (both macro and micro), can make yeast to perform better than ever. In this study, it has been found that, 0.1% NaCl, 0.01% CaCl 2, 0.125% KH 2PO 4, 0.125% K 2HPO 4, 0.05% MgSO 4.7H 2O, 1% Na 2HPO 4.2H 2O, 1 mg/L boric acid, 10 μg/ ml each of Fe 2+, Mn 2+ and Zn 2+ is the optimum requirement for our organism to produce the maximum amount of acetic acid i.e. 10.678 g/L.