Different types of auxin like IAA, NAA and 2, 4-D in combination with kinetin have been used for the initiation and maintenance of tissues of Nigella sativa. Both the changes in growth pattern and cytological behaviour have been observed in all the cases. Chromosomal abnormalities have been found to occur in maximum frequency where 2, 4-D is present in the media with kinetin. © 1980 Taylor & Francis Group, LLC.