This study was directed to identify early life-history stages of eight culturable species currently under threat through DNA barcodes. A total of 22 sequences of cytochrome c oxidase I gene were generated from eight species using multiple specimens producing 655 nucleotide base pairs per taxon. The neighbourjoining tree showed three major clusters for three different orders. Similar species were clustered under the same nodes and dissimilar species were clustered under separate nodes signifying species specificity. This can be utilized for accurate identification of threatened fish species and in turn assist in conservation of fish stock either inside freshwater protected areas or in the river systems.