Dietary protein variation has been found to alter brain regional neurochemistry with aging. In the present investigation, we studied the effect of short-term treatment of protein-carbohydrate variable diet to rat on hypothalamic and hippocampal γ-amino butyric acid (GABA)-glutamate metabolism with increase of age. Exposure of male albino rats with diet containing normal protein (20%)-normal carbohydrate (68%) increased GABA metabolism and decreased glutamate metabolism in both hypothalamus and hippocampus with the increase of age. GABA-glutamate metabolism of rats having low protein (8%)-high carbohydrate (80%) diet for short-term period (STP), was activated in young age (3 months) and decreased in old age (18 months) in both the brain regions. On the contrary, intake of high protein (50%)-low carbohydrate (38%) diet under similar condition decreased GABA-glutamate metabolism in both hypothalamus and hippocampus of young brain and increased only in hypothalamus of aged brain. In hippocampus of aged brain the same diet decreased glutamate metabolism without changing its GABA metabolism. These results suggest that an age-associated change in GABA-glutamate metabolism depends on the amount of dietary protein and carbohydrate and also on the brain region.

S PAL

M SEN

M K PODDAR

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Dietary variation of protein–carbohydrate: Effect on hypothalamic and hippocampal GABA–glutamate in relation to aging

Nutritional Neuroscience

Glutamatergic activity of hypothalamus and hippocampus of young (3 months) male albino rats having normal diet [protein (20%)-carbohydrate (68%)] was increased with the increase of age. Long-term (60 consecutive days) feeding of low protein (8%)-high carbohydrate (80%) diet (LP-HC) increased glutamatergic activity in these brain regions of young rats and decreased that in aged (18 months). On the contrary, supplementation of high protein (50%)-low carbohydrate (38%) diet (HP-LC) under similar condition decreased glutamatergic activity in those brain regions of young and increased that in aged brain regions. Thus, prolonged exposure of LP-HC diet may damage young brain; whereas, HP-LC diet under similar condition causes excitotoxicity to aged brain. Therefore, considering the present scenario in relation to metabolism and receptor activity of glutamatergic system, it may be suggested that long-term consumption of LP-HC and HP-LC diets modulate the brain regional glutamatergic activity reversibly with age. © 2007 Springer Science+Business Media, LLC.

Neurochemical Research

Long-term exposure of variable dietary protein-to-carbohydrate ratio: Effect on brain regional glutamatergic activity with age

Manipulation of dietary protein has been found to be the most useful dictator in the age-associated decline of neuroimmune activity in mammals. In the present study, we sought to clarify the effect of dietary protein on age-induced alterations of hypothalamic glutamatergic activity and immune response. The hypothalamic glutamatergic activity and immune response were found to increase and decrease, respectively, with the increase in age of rats from young (3 months) to old (18 months) maintained with normal (20%) protein diet. Intake of low (5%) protein diet (LPD) and high (40%) protein diet (HPD) under short-term period (7 days) failed to alter the age-associated loss of immune response and increase in hypothalamic glutamatergic activity. However, long-term (30 days) supplementation of LPD retarded the age-induced decline in immune response and increase in hypothalamic glutamatergic activity, whereas, HPD consumption under similar condition potentiated the age-related immunosuppression and increase in hypothalamic glutamatergic activity. These results suggest that (a) the age-associated immunosuppression may be inversely related to the hypothalamic glutamatergic activity and (b) consumption of diets having variable quantity of protein without variation of calorie content modulates immune response and hypothalamic glutamatergic activity depending upon age and duration of dietary supplementation.

Is age-induced decline in immune response associated with hypothalamic glutamate receptor density and dietary protein?

Single administration of aldrin (2-10 mg/kg) to adult male albino rats (120-130 g) enhanced locomotor activity (LA), with the maximum effect reached 2 h after treatment. The measurement of steady state levels of glutamate, glutamine and the activities of their metabolizing enzymes in different regions of the brains of rats treated with aldrin under its nontolerant condition showed that aldrin enhanced the activity of the neuronal glutamate system in the cerebral cortex, cerebellum and hypothalamus. Moreover, treatment with the glumatergic NMDA receptor antagonist D,L-2-amino-7-phosphonoheptanoic acid, in the absence and presence of aldrin, reduced the LA of control rats and attenuated the aldrin-induced increase in LA of treated rats. These results suggest that aldrin-induced activation of the central glutamate system may be a cause of stimulation of LA with aldrin under its nontolerant condition. © 2001 Prous Science. All rights reserved.

Methods and Findings in Experimental and Clinical Pharmacology

Aldrin-induced stimulation of locomotor activity and brain regional glutamate

The effect of dietary protein on hypothalamic GABAergic activity and immune response of rats in relation to age was studied. The age-induced (due to increase of age from three to 18 months) decrease in hypothalamic GABAergic activity and immune response were potentiated with the supplementation of protein rich diet under both short- and long-term conditions. Long-term consumption of protein-poor diet, in contrast, produced activation of hypothalamic GABAergic activity with an immunopotentiation with the increase of age from three to 18 months; whereas, short-term supplementation of low protein diet did not show any effect.The results of the present study may indicate that the activation or inhibition of hypothalamic GABAergic activity by immunopotentiation or immunosuppression during aging depends on the variation of the amount of dietary protein as well as the duration of its supplementation. Copyright (C) 2000 IBRO.

Neuroscience

Dietary protein alters age-induced change in hypothalamic GABA and immune response

Short-term supplementation of low protein diet (LPD) or high protein diet (HPD) to the aged (18 months old) rats did not change the hypothalamic GABAergic activity and immune response. Long-term supplementation of LPD on the other hand, enhanced hypothalamic GABAergic activity with an immunopotentiation by increasing the T-cell proliferating activity and peripheral blood lymphocyte count in the same age group of rats. Unlike LPD, HPD under long-term supplementation to the aged rats reduced the hypothalamic GABAergic activity with immunosuppression. These results thus suggest that (i) hypothalamic GABAergic activity is a regulatory index of immune response in aged mammals and (ii) long-term consumption of protein-poor or protein-rich diet may be an exogenous modulator of neuroimmune regulation in relation to central GABAergic activity and immune response.

Journal	Nutritional Neuroscience
Publisher	MANEY PUBLISHING
ISSN	1028-415X